Enzyme Exercise Review

1. Using the spectrophotometer, were the absorbency readings directly or indirectly proportional to the concentration of product in the reaction mixtures:

_________________________________________

2. In the experiment on varying the amount of enzyme concentration, would you expect the plot to continue upward, level off (stops) or go downward if excess enzyme were added?

_________________________________________

3. What would have happened, if you had omitted substrate from the reaction mixture? ____________________________________

4. What is the relationship between pH and the rate of substrate breakdown?

_______________________________________________________________________

5. What was the pH optimum for alkaline phosphatase? __________________

6. Why was NaOH added to the reaction mixture? ________________________

7. In the ‘effect of temperature experiment’, what was the effect of temperatures greater than 50 degrees?

________________________________________________________________________

8. In general, what is the relationship between temperature and the rate of substrate breakdown? _______________________________________________

9. If a reaction involved the following steps: , how many enzymes would have been involved?
_____________________

10. What part of the enzyme makes it so sensitive to environmental factors such as pH and temperature.